Journal: Frontiers in Cell and Developmental Biology
Article Title: Influence of secretome from porcine cardiosphere-derived cells on porcine macrophage polarization and their possible implications for cardiac remodeling post-myocardial infarction in vitro
doi: 10.3389/fcell.2025.1601743
Figure Lengend Snippet: Workflow of materials and methods. (A) Isolation and bioinformatic analysis of secretome from CDCs. (B) Differentiation and treatment of macrophages with S-CDCs (C) Functional assays with conditioned medium from macrophages: (C1) Interaction with endothelial cells and (C2) interaction with cardiac fibroblasts. CDCs: cardiosphere-derived cells; S-CDCs: secretome released by cardiosphere-derived cells. HUVECs: Human umbilical vein endothelial cells. PCF: porcine cardiac fibroblasts. This figure was created with BioRender.com .
Article Snippet: HUVECs (ATCC) were cultured in Endothelial Cell Basal Medium (EBM-2, Lonza) supplemented with growth factors (EGM-2 SingleQuotsTM, Lonza) and used at a maximum passage number of five.
Techniques: Isolation, Functional Assay, Derivative Assay